4V4M

1.45 Angstrom Structure of STNV coat protein

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.934
Spacegroup name	C 1 2 1
Unit cell lengths	307.585, 302.265, 181.923
Unit cell angles	90.00, 92.77, 90.00

Refinement procedure

Resolution	12.000 - 1.450
R-factor	0.17633
Rwork	0.175
R-free	0.20761
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2STV
RMSD bond length	0.009
RMSD bond angle	1.220
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	1.450
Rmerge	0.100
Number of reflections	2217504
<I/σ(I)>	3.6
Completeness [%]	80.2
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	0.4 % PEG 6000, pH 6.2, 1 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K