4V4G

Crystal structure of five 70s ribosomes from Escherichia Coli in complex with protein Y.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	110
Detector technology	CCD
Collection date	2004-03-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	687.900, 687.900, 1933.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 11.500
R-factor	0.395
Rwork	0.395
R-free	0.40100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1PNS 1PNU
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	11.800
High resolution limit [Å]	11.500	11.500
Number of reflections	62383
<I/σ(I)>	7.4	2
Completeness [%]	93.9	77.9
Redundancy	6.1	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		18-22% 2-methyl-2,4-pentanediol (MPD), 0-5% ethanol, 20-25mM MgCl2, 200-320mM NH4Cl, 1mM spermine, 0-0.5mM spermidine, pH 6.0-6.5, pH 6.50
1		6.5		18-22% 2-methyl-2,4-pentanediol (MPD), 0-5% ethanol, 20-25mM MgCl2, 200-320mM NH4Cl, 1mM spermine, 0-0.5mM spermidine, pH 6.0-6.5, pH 6.50
1		6.5		18-22% 2-methyl-2,4-pentanediol (MPD), 0-5% ethanol, 20-25mM MgCl2, 200-320mM NH4Cl, 1mM spermine, 0-0.5mM spermidine, pH 6.0-6.5, pH 6.50

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
10	1	2	MPD
2	1	1	ethanol
3	1	1	MgCl2
4	1	1	NH4Cl
5	1	1	spermine
6	1	1	spermidine
7	1	1	H2O
8	1	2	MgCl2
9	1	2	NH4Cl