4V4A
Crystal Structure of the Wild Type Ribosome from E. Coli 70S Ribosome.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2000-12-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0, 1.1 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 683.890, 683.890, 386.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.000 - 9.500 |
Rwork | 0.389 |
R-free | 0.40700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j5e 1LNR |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 300.000 | 9.680 |
High resolution limit [Å] | 9.500 | 9.500 |
Rmerge | 0.142 | 0.549 |
Number of reflections | 26821 | |
<I/σ(I)> | 44.8 | 2.5 |
Completeness [%] | 92.1 | 71.8 |
Redundancy | 25.1 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | MPD, ethanol, magnesium chloride, ammonium chloride, spermine, spermidine, MES , pH 6.5, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | magnesium chloride | ||
11 | 1 | 2 | ammonium chloride | ||
12 | 1 | 2 | H2O | ||
2 | 1 | 1 | ethanol | ||
3 | 1 | 1 | magnesium chloride | ||
4 | 1 | 1 | ammonium chloride | ||
5 | 1 | 1 | spermine | ||
6 | 1 | 1 | spermidine | ||
7 | 1 | 1 | MES | ||
8 | 1 | 1 | H2O | ||
9 | 1 | 2 | MPD |