4V49
Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli 70S Ribosome.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-08-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 682.320, 682.320, 386.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 8.700 |
| Rwork | 0.394 |
| R-free | 0.41500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1J5E 1LNR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 175.000 | 8.900 |
| High resolution limit [Å] | 8.700 | 8.700 |
| Number of reflections | 36338 | |
| <I/σ(I)> | 9 | 2.5 |
| Completeness [%] | 98.5 | 99.2 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | MPD, ethanol, magnesium chloride, ammonium chloride, spermine, spermidine, MES, pH 6.5, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | magnesium chloride | ||
| 11 | 1 | 2 | ammonium chloride | ||
| 12 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | ethanol | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | ammonium chloride | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 1 | spermidine | ||
| 7 | 1 | 1 | MES | ||
| 8 | 1 | 1 | H2O | ||
| 9 | 1 | 2 | MPD |
