4V43
Structural and mechanistic basis for allostery in the bacterial chaperonin GroEL
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.666, 264.235, 294.799 |
| Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.520 |
| Rwork | 0.291 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kp8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.114 | 0.337 |
| Number of reflections | 217987 | |
| <I/σ(I)> | 1.396 | |
| Completeness [%] | 85.1 | 51.4 |
| Redundancy | 11.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
