4V41
E. COLI (LAC Z) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-MONOCLINIC)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-03-01 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.900, 207.500, 509.900 |
| Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| Rwork | 0.199 |
| R-free | 0.20700 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.700 |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.070 |
| Number of reflections | 559917 |
| Completeness [%] | 73.0 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | PEG 8000, Cacodylate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
