4V3Q
Designed armadillo repeat protein with 4 internal repeats, 2nd generation C-cap and 3rd generation N-cap.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 32 |
| Unit cell lengths | 96.498, 96.498, 96.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.340 - 1.800 |
| R-factor | 0.19168 |
| Rwork | 0.189 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4db6 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.828 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.340 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.886 |
| Number of reflections | 93024 | |
| <I/σ(I)> | 12.6 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25 % PEG 2KMME 0.2 M CA-ACETATE 0.1 M NA-ACETATE, PH 5.5 |
