4V3O
Designed armadillo repeat protein with 5 internal repeats, 2nd generation C-cap and 3rd generation N-cap.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 102.586, 102.586, 111.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.110 - 2.000 |
| R-factor | 0.17065 |
| Rwork | 0.168 |
| R-free | 0.22426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4db6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.711 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.550 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.750 |
| Number of reflections | 76669 | |
| <I/σ(I)> | 12.2 | 2.6 |
| Completeness [%] | 94.3 | 94.3 |
| Redundancy | 7.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15 % PEG 4000 0.2 M CA-ACETATE 0.1 M NA-ACETATE, PH 5.5 |
