4V3M
Membrane bound pleurotolysin prepore (TMH2 helix lock) trapped with engineered disulphide cross-link
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.3 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 154 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 17 |
| Software | Flex-EM,MODELLER,TEMPy,UCSF Chimera,IMAGIC,SPIDER |
| Symmetry | C13 |
