4V3J
Structural and functional characterization of a novel monotreme- specific protein from the milk of the platypus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-23 |
| Detector | ADXV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.351, 73.008, 56.732 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 57.270 - 1.970 |
| R-factor | 0.17401 |
| Rwork | 0.172 |
| R-free | 0.21148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.576 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.080 | 0.740 |
| Number of reflections | 26295 | |
| <I/σ(I)> | 22.2 | 2.7 |
| Completeness [%] | 99.1 | 97.8 |
| Redundancy | 11.2 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.65 | 281 | PROTEIN WAS 4.7 MG/ML AND SET UP IN 200 PLUS 200 NL DROPS AT 8C WITH: 48 MM NACL, 28.3% PEG 8K, 10% MALONATE-IMIDAZOLE-BORATE BUFFER AT PH 8.65. |
