4V3I
Crystal Structure of TssL from Vibrio cholerae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2013-06-04 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 |
| Unit cell lengths | 78.361, 78.361, 49.456 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.979 - 1.499 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.19390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u66 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.191 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 27803 | |
| <I/σ(I)> | 59 | 6.63 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 11 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 1.8 M SODIUM CHLORIDE, 0.1 M SODIUM POTASSIUM PHOSPHATE PH 6.4 |
