4V3H
Crystal structure of CymA from Klebsiella oxytoca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.211, 61.430, 108.237 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 43.353 - 1.830 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v3g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 77325 | |
| <I/σ(I)> | 13.8 | 2 |
| Completeness [%] | 97.0 | 93 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 23% PEG2000 0.1 M TRIS PH 8 0.3 M MAGNESIUM NITRATE HEXAHYDRATE |
