4V3C
The structure of alpha2,3-sialyltransferase variant 2 from Pasteurella dagmatis in complex with the donor product CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 111.750, 111.750, 65.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.000 - 1.840 |
| R-factor | 0.17503 |
| Rwork | 0.173 |
| R-free | 0.21796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2u |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 0.770 |
| Number of reflections | 44015 | |
| <I/σ(I)> | 18.8 | 1.8 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 MG/ML PROTEIN CONCENTRATION 0.1M SODIUM CITRATE, 20% W/V PEG 3000, PH 5.5 |
