4V39
Apo-structure of alpha2,3-sialyltransferase variant 2 from Pasteurella dagmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.190, 56.850, 79.680 |
| Unit cell angles | 90.00, 111.35, 90.00 |
Refinement procedure
| Resolution | 74.210 - 2.600 |
| R-factor | 0.19463 |
| Rwork | 0.191 |
| R-free | 0.26320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.300 | 2.520 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.130 | 0.810 |
| Number of reflections | 22648 | |
| <I/σ(I)> | 10.9 | 2.1 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 MG/ML PROTEIN CONCENTRATION 0.1M CITRATE, 20%W/V PEG 6000, PH 5.0 |
