4V36
The structure of L-PGS from Bacillus licheniformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 46.890, 66.260, 71.150 |
Unit cell angles | 112.43, 94.07, 98.94 |
Refinement procedure
Resolution | 37.337 - 2.100 |
R-factor | 0.1998 |
Rwork | 0.197 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.541 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.340 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.100 | 0.600 |
Number of reflections | 36408 | |
<I/σ(I)> | 8.7 | 2.1 |
Completeness [%] | 80.6 | 19 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.2 | 0.2 M NACL; 0.1 M PHOSPHATE-CITRATE PH 4.2; 10%(W/V) GLYCEROL; 0.5 MM L-LYSINE AMIDE; PRIOR TO CRYO-COOLING, 30% (V/V) WERE ADDED AS A CRYOPROTECTANT |