4V36
The structure of L-PGS from Bacillus licheniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.890, 66.260, 71.150 |
| Unit cell angles | 112.43, 94.07, 98.94 |
Refinement procedure
| Resolution | 37.337 - 2.100 |
| R-factor | 0.1998 |
| Rwork | 0.197 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.541 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.340 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 36408 | |
| <I/σ(I)> | 8.7 | 2.1 |
| Completeness [%] | 80.6 | 19 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 0.2 M NACL; 0.1 M PHOSPHATE-CITRATE PH 4.2; 10%(W/V) GLYCEROL; 0.5 MM L-LYSINE AMIDE; PRIOR TO CRYO-COOLING, 30% (V/V) WERE ADDED AS A CRYOPROTECTANT |
