4V35
The Structure of A-PGS from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 94.180, 94.180, 166.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.695 - 2.300 |
| R-factor | 0.1758 |
| Rwork | 0.175 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.160 | 1.340 |
| Number of reflections | 33909 | |
| <I/σ(I)> | 12.34 | 2.01 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 7.8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 0.085 M NAAC PH 6.4, 1.6 M (NH4)2SO4, 20% (V/V) GLYCEROL |
