4V31
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Deoxyuridine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.809, 59.993, 88.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.410 - 1.800 |
R-factor | 0.16656 |
Rwork | 0.164 |
R-free | 0.21855 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.019 |
RMSD bond angle | 1.868 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.400 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.500 |
Number of reflections | 28709 | |
<I/σ(I)> | 8.71 | 2.33 |
Completeness [%] | 98.8 | 97.4 |
Redundancy | 2.87 | 2.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M CITRIC ACID PH 3.5, 25 %(W/V) PEG 3350 |