4V2S
Crystal structure of Hfq in complex with the sRNA RydC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.940, 73.356, 137.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.320 - 3.480 |
| R-factor | 0.21989 |
| Rwork | 0.217 |
| R-free | 0.28158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rer |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.470 | 3.690 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.150 | 0.890 |
| Number of reflections | 9190 | |
| <I/σ(I)> | 9.6 | 2.3 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M TRI-SODIUM CITRATE, 0.1 M SODIUM CACODYLATE (PH 6.5), 15% ISOPROPANOL |
