4V2E
FLRT3 LRR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.810, 61.970, 92.970 |
| Unit cell angles | 90.00, 106.38, 90.00 |
Refinement procedure
| Resolution | 57.380 - 2.500 |
| R-factor | 0.3451 |
| Rwork | 0.345 |
| R-free | 0.35140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.460 | |
| Number of reflections | 63975 | |
| <I/σ(I)> | 3.1 | 0.98 |
| Completeness [%] | 90.4 | 89.2 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
