4V2C
mouse FLRT2 LRR domain in complex with rat Unc5D Ig1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.140, 150.910, 67.220 |
| Unit cell angles | 90.00, 102.79, 90.00 |
Refinement procedure
| Resolution | 87.840 - 4.000 |
| R-factor | 0.3191 |
| Rwork | 0.318 |
| R-free | 0.33490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.170 | |
| Number of reflections | 102048 | |
| <I/σ(I)> | 8.4 | 0.87 |
| Completeness [%] | 98.8 | 87.8 |
| Redundancy | 6.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
