4V2A

human Unc5A ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Detector	PILATUS
Spacegroup name	P 21 21 21
Unit cell lengths	25.830, 87.770, 133.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.880 - 2.400
R-factor	0.3276
Rwork	0.327
R-free	0.34360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	0.850
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.110
Number of reflections	11029
<I/σ(I)>	13.88	0.61
Completeness [%]	86.8	46.5
Redundancy	6.6	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1