4V29

The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	173
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	34.848, 88.137, 108.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.300 - 1.600
R-factor	0.1955
Rwork	0.194
R-free	0.21880
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	1.092
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.280
High resolution limit [Å]	1.600
Rmerge	0.040
Number of reflections	84856
<I/σ(I)>	37.9
Completeness [%]	99.4
Redundancy	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% PEG 6K, 0.1 MES PH6, 0.2 M LICL