4V23
RSV Matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-29 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.294, 79.156, 65.945 |
| Unit cell angles | 90.00, 96.24, 90.00 |
Refinement procedure
| Resolution | 65.554 - 1.700 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.21110 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.500 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.290 |
| Number of reflections | 29385 | |
| <I/σ(I)> | 16.1 | 2.9 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 4.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% W/V PEG 8000, 20% V/V ETHYLENE GLYCOL, 0.02 M EACH OF SODIUM FORMATE, AMMONIUM ACETATE, TRISODIUM CITRATE, SODIUM POTASSIUM TARTRATE, 0.1 M MOPS/HEPES-NA (7.5) |
