4V20
The 3-D structure of the cellobiohydrolase, Cel7A, from Aspergillus fumigatus, disaccharide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Collection date | 2011-08-10 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.560, 130.780, 46.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.490 - 1.500 |
| R-factor | 0.13334 |
| Rwork | 0.132 |
| R-free | 0.15829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v1z |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.702 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.490 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 73170 | |
| <I/σ(I)> | 19.8 | 2.9 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG6K, 10 MM ZNCL2, MES PH 6.0, SEEDING OF LIGAND COMPLEX DROPS WITH NATIVE SEEDS |
