4V1L
High resolution structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-31 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 102.250, 102.525, 109.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.830 - 1.750 |
| R-factor | 0.15956 |
| Rwork | 0.158 |
| R-free | 0.17975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v1k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.455 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.190 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.130 | 1.380 |
| Number of reflections | 58166 | |
| <I/σ(I)> | 15.8 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1 M SODIUM CITRATE, 0.1 M MES PH 6.5, 30% GLYCEROL WAS ADDED IN ABOVE CONDITOIN FOR TEH CRYOPROTECTANT |
