4V1B
Structure of a novel carbohydrate binding module from glycoside hydrolase family 5 glucanase from Ruminococcus flavefaciens FD-1 collected at the Zn edge
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 132.260, 132.260, 104.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.130 - 2.690 |
| R-factor | 0.19451 |
| Rwork | 0.194 |
| R-free | 0.20339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v18 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.992 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.130 | 2.760 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.150 | 1.500 |
| Number of reflections | 9947 | |
| <I/σ(I)> | 14.1 | 3.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 19.3 | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2 M K2SO4, 20% PEG 3350. CRYO 30% GLYCEROL IN THE ABOVE CONDITION, PH 7.0 |
