4V17
Structure of a novel carbohydrate binding module from glycoside hydrolase family 5 glucanase from Ruminococcus flavefaciens FD-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-06 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 131.093, 131.093, 105.199 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.160 - 2.000 |
R-factor | 0.20403 |
Rwork | 0.203 |
R-free | 0.22875 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SEMET DERIVATIVE FROM 2.28 A |
RMSD bond length | 0.009 |
RMSD bond angle | 1.263 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.170 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.120 | 1.500 |
Number of reflections | 26385 | |
<I/σ(I)> | 12.5 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 16.9 | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 1.2 M NA2 CITRATE, 0.1 M HEPES PH 7.5 CRYO 30% GLYCEROL IN ABOVE CONDITION |