4V15
Crystal structure of D-threonine aldolase from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.267, 84.278, 72.852 |
| Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
| Resolution | 35.759 - 1.500 |
| R-factor | 0.1499 |
| Rwork | 0.148 |
| R-free | 0.17670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3GWQ AND 3LLX |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.356 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.650 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 111114 | |
| <I/σ(I)> | 6.3 | 1.6 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.1 | 13% (W/V) PEG-3350, 200 MM SODIUM CHLORIDE, 100 MM TRIS/HCL, PH 8.1 |
