4V15
Crystal structure of D-threonine aldolase from Alcaligenes xylosoxidans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-16 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.267, 84.278, 72.852 |
Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
Resolution | 35.759 - 1.500 |
R-factor | 0.1499 |
Rwork | 0.148 |
R-free | 0.17670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3GWQ AND 3LLX |
RMSD bond length | 0.019 |
RMSD bond angle | 1.356 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.650 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.080 | 0.330 |
Number of reflections | 111114 | |
<I/σ(I)> | 6.3 | 1.6 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.1 | 13% (W/V) PEG-3350, 200 MM SODIUM CHLORIDE, 100 MM TRIS/HCL, PH 8.1 |