4V14

Structure and function analysis of MutT from the psychrofile fish pathogen Aliivibrio salmonicida and the mesophile Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Collection date	2008-03-03
Spacegroup name	P 41 21 2
Unit cell lengths	81.150, 81.150, 90.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.001 - 2.420
R-factor	0.2302
Rwork	0.226
R-free	0.30510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a6v
RMSD bond length	0.018
RMSD bond angle	1.708
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	250.000	2.550
High resolution limit [Å]	2.420	2.420
Rmerge	0.050	0.600
Number of reflections	11909
<I/σ(I)>	21.1	2.8
Completeness [%]	99.1	100
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.2 M LITHIUM SULPHATE, 0.1 M TRIS PH 8.5, 1.26 M AMMONIUM SULPHATE