4V0V
The crystal structure of mouse PP1G in complex with truncated human PPP1R15B (631-660)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.800, 67.890, 156.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.263 - 1.610 |
| R-factor | 0.1776 |
| Rwork | 0.176 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fjm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CCP4I |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.260 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.080 | 0.810 |
| Number of reflections | 92584 | |
| <I/σ(I)> | 12.5 | 3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.3 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.4M SODIUM MALONATE, PH7.0 |
