4V0K
Crystal structure of the CrARL6DN in the GDP bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.725, 65.725, 185.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.504 - 1.438 |
| R-factor | 0.1562 |
| Rwork | 0.155 |
| R-free | 0.18390 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.030 | 0.600 |
| Number of reflections | 83301 | |
| <I/σ(I)> | 17.8 | 1.8 |
| Completeness [%] | 96.2 | 89.9 |
| Redundancy | 8.8 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% POLYETHYLENE GLYCOL 3350, 0.1M HEPES PH7.5, 5MM CDCL2, 5MM MGCL2, 5MM NICL2, 5MM COCL2 |
