4V0B
Escherichia coli FtsH hexameric N-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 6 |
| Unit cell lengths | 119.110, 119.110, 34.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.988 - 2.550 |
| R-factor | 0.2102 |
| Rwork | 0.207 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2muy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.700 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.060 | 0.780 |
| Number of reflections | 9209 | |
| <I/σ(I)> | 15.6 | 2.01 |
| Completeness [%] | 97.4 | 86.5 |
| Redundancy | 5.12 | 3.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M AMMONIUM SULFATE, 0.1 M HEPES PH 7.5 |
