4UZZ

Crystal structure of the TtIFT52-46 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-21
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 32 2 1
Unit cell lengths	84.394, 84.394, 95.477
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.197 - 2.318
R-factor	0.2406
Rwork	0.239
R-free	0.26350
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.219
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.400
High resolution limit [Å]	2.320	2.320
Rmerge	0.070	1.500
Number of reflections	17160
<I/σ(I)>	17	0.6
Completeness [%]	99.0	92.9
Redundancy	6.2	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2		50MM TRISD PH 8.2, 0.6M TRI-SODIUM- CITRATE