4UZK

STRUCTURE OF THE WNT DEACYLASE NOTUM FROM DROSOPHILA - CRYSTAL FORM II - 1.9A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-15
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	59.252, 93.103, 142.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.880 - 1.900
R-factor	0.17677
Rwork	0.176
R-free	0.20365
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.594
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	142.000
High resolution limit [Å]	1.900
Rmerge	0.080
Number of reflections	62554
<I/σ(I)>	13.9
Completeness [%]	99.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.5		0.1 M NACL, 0.1 M LI2SO4, 0.1 M CAPSO PH 9.5, 12.0 %W/V PEG 4000