4UZJ
STRUCTURE OF THE WNT DEACYLASE NOTUM FROM DROSOPHILA - CRYSTAL FORM I - 2.4A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-28 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.388, 81.394, 86.945 |
Unit cell angles | 90.00, 105.54, 90.00 |
Refinement procedure
Resolution | 83.770 - 2.400 |
R-factor | 0.19842 |
Rwork | 0.197 |
R-free | 0.24338 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.494 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.050 |
Number of reflections | 30929 |
<I/σ(I)> | 13.8 |
Completeness [%] | 98.9 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.12 M ALCOHOLS, 0.1 M TRIS/BICINE 8.5, 30 % EDO_P8K |