4UZJ
STRUCTURE OF THE WNT DEACYLASE NOTUM FROM DROSOPHILA - CRYSTAL FORM I - 2.4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-28 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.388, 81.394, 86.945 |
| Unit cell angles | 90.00, 105.54, 90.00 |
Refinement procedure
| Resolution | 83.770 - 2.400 |
| R-factor | 0.19842 |
| Rwork | 0.197 |
| R-free | 0.24338 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.050 |
| Number of reflections | 30929 |
| <I/σ(I)> | 13.8 |
| Completeness [%] | 98.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.12 M ALCOHOLS, 0.1 M TRIS/BICINE 8.5, 30 % EDO_P8K |
