4UZG
Crystal structure of group B streptococcus pilus 2b backbone protein SAK_1440
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-24 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.640, 53.920, 54.770 |
Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
Resolution | 47.633 - 1.060 |
R-factor | 0.1256 |
Rwork | 0.125 |
R-free | 0.14220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3ICH 3qdh 2h9g |
RMSD bond length | 0.010 |
RMSD bond angle | 1.416 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.630 | 1.090 |
High resolution limit [Å] | 1.060 | 1.060 |
Rmerge | 0.034 | 0.250 |
Number of reflections | 119342 | |
<I/σ(I)> | 17 | 2.98 |
Completeness [%] | 95.2 | 88.2 |
Redundancy | 2.6 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.2M CALCIUM ACETATE, 20% PEG 3350, pH 8.0 |