4UZG

Crystal structure of group B streptococcus pilus 2b backbone protein SAK_1440

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-24
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	47.640, 53.920, 54.770
Unit cell angles	90.00, 90.97, 90.00

Refinement procedure

Resolution	47.633 - 1.060
R-factor	0.1256
Rwork	0.125
R-free	0.14220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3ICH 3qdh 2h9g
RMSD bond length	0.010
RMSD bond angle	1.416
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.630	1.090
High resolution limit [Å]	1.060	1.060
Rmerge	0.034	0.250
Number of reflections	119342
<I/σ(I)>	17	2.98
Completeness [%]	95.2	88.2
Redundancy	2.6	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		0.2M CALCIUM ACETATE, 20% PEG 3350, pH 8.0