4UZF
R66E mutant of FAD synthetase from Corynebacterium ammoniagenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 133.834, 133.834, 133.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.520 |
| R-factor | 0.20294 |
| Rwork | 0.200 |
| R-free | 0.25167 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.610 | 2.650 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 26991 | |
| <I/σ(I)> | 19.4 | 3.7 |
| Completeness [%] | 98.5 | 89.8 |
| Redundancy | 8.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5 M LI2SO4 AND 0.1 M HEPES-NAOH PH 7.5 |
