4UZE
R66A mutant of FAD synthetase from Corynebacterium ammoniagenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 133.611, 133.611, 133.611 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.340 |
| R-factor | 0.22012 |
| Rwork | 0.218 |
| R-free | 0.25575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.540 | 2.460 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 33765 | |
| <I/σ(I)> | 21.4 | 3.9 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 10.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5 M LI2SO4 AND 0.1 M HEPES-NAOH PH 7.5 |
