4UZC

KSHV LANA (ORF73) C-terminal domain, spiral: hexagonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-19
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65 2 2
Unit cell lengths	103.180, 103.180, 228.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	89.360 - 3.700
R-factor	0.20278
Rwork	0.201
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ypy
RMSD bond length	0.001
RMSD bond angle	0.508
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (PHASER-MR)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.360	3.840
High resolution limit [Å]	3.700	3.700
Rmerge	0.230
Number of reflections	7874
<I/σ(I)>	18.53	1.92
Completeness [%]	95.9	99.1
Redundancy	29.7	31.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.4 UL OF 31.5 MG/ML PROTEIN IN 5 MM BISTRIS-CL, 5 MM DTT, PH 6.5 WERE AS SUCH EQUILIBRATED AGAINST A RESERVOIR SOLUTION OF 170 MM LITHIUM ACETATE AND 18% (W/V) PEG3350 IN A SITTING DROP SETUP AT 20 DEGREE CENTIGRADE. AFTER TWO DAYS, CRYSTALS WERE DETACHED FROM THE CARRIER PLASTIC BY ADDING 1 UL OF 2 M AMMONIUM FORMATE.