4UZ5

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM IV - 2.1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-10
Detector	DECTRIS PILATUS
Spacegroup name	P 41 3 2
Unit cell lengths	138.416, 138.416, 138.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.870 - 2.100
R-factor	0.18936
Rwork	0.187
R-free	0.23710
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.504
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	69.000
High resolution limit [Å]	2.100
Rmerge	0.110
Number of reflections	27077
<I/σ(I)>	18.3
Completeness [%]	100.0
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		10 %W/V PEG4000, 0.01 M CACL2, 0.05 M NACACOD PH 6.0, 0.20 M KCL, 1 MM HEPARIN HEXAMER