4UZ3
Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-27 |
Detector | MARRESEARCH |
Spacegroup name | P 21 3 |
Unit cell lengths | 105.490, 105.490, 105.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.589 - 1.750 |
R-factor | 0.1538 |
Rwork | 0.152 |
R-free | 0.18870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.915 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.010 | 0.870 |
Number of reflections | 39631 | |
<I/σ(I)> | 20.3 | 3.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.3 | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M MES PH6.5, 1.6 M AMMONIUM SULFATE, 5% DIOXANE |