4UZ2

Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Collection date	2013-11-27
Spacegroup name	P 42 21 2
Unit cell lengths	122.930, 122.930, 76.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.796 - 2.500
R-factor	0.2163
Rwork	0.212
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.002
RMSD bond angle	0.549
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.810
Number of reflections	20666
<I/σ(I)>	24.14	3.2
Completeness [%]	98.8	99.8
Redundancy	11.7	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				28& PEG MME 2000, 0.1M POTASSIUM THIOCYANATE