4UZ0

Crystal Structure of apoptosis repressor with CARD (ARC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	295
Detector technology	CCD
Collection date	2014-07-12
Detector	ADSC QUANTUM 315r
Spacegroup name	P 65
Unit cell lengths	99.351, 99.351, 51.102
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.436 - 2.399
R-factor	0.1731
Rwork	0.170
R-free	0.20010
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.080	0.590
Number of reflections	11384
<I/σ(I)>	45.7	5.7
Completeness [%]	99.6	100
Redundancy	7.2	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2		0.1 M IMIDAZOLE PH 8.2, 0.6 M DIAMMONIUM PHOSPHATE