4UYT
X-ray structure of the N-terminal domain of the flocculin Flo11 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.430, 54.850, 120.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.540 - 1.030 |
| R-factor | 0.12882 |
| Rwork | 0.129 |
| R-free | 0.14416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOMR) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.460 | 1.090 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.030 | 0.380 |
| Number of reflections | 98507 | |
| <I/σ(I)> | 18.7 | 2.6 |
| Completeness [%] | 98.5 | 93.6 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PROTEIN WAS CRYSTALLIZED FROM 100 MM NA3CITRATE2H2O, PH 6.0, 1 M LI2SO4, 4% PEG 4000; THEN SOAKED IN THIS CONDITION CONTAINING 25 % GLYCERIN ADDITIONALLY FOR CRYOPROTECTION. |
