4UYR
X-ray structure of the N-terminal domain of the flocculin Flo11 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.450, 54.830, 121.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.760 - 0.890 |
| R-factor | 0.12699 |
| Rwork | 0.127 |
| R-free | 0.13399 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.760 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXCDE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.760 | 0.940 |
| High resolution limit [Å] | 0.890 | 0.890 |
| Rmerge | 0.060 | 0.780 |
| Number of reflections | 156350 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 30% PEG 400, 100 MM MGCL2, 100 MM NAHEPES, PH 7.5. |
