4UYK
Crystal structure of a Signal Recognition Particle Alu domain in the elongation arrest conformation
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.860, 100.860, 197.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.820 - 3.220 |
| R-factor | 0.19365 |
| Rwork | 0.192 |
| R-free | 0.23315 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 30821 | |
| <I/σ(I)> | 9.09 | 1.74 |
| Completeness [%] | 97.8 | 95.9 |
| Redundancy | 2.18 | 2.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 17.5% PEG 400, 5% GLYCEROL BUFFERED IN 100 MM SODIUM ACETATE PH 5.0 |
