4UYJ
Crystal structure of a Signal Recognition Particle Alu domain in the elongation arrest conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.950, 108.600, 128.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.760 - 3.350 |
| R-factor | 0.19455 |
| Rwork | 0.192 |
| R-free | 0.23643 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SRP914 ALU134 MODEL |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.943 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.101 | 0.770 |
| Number of reflections | 21235 | |
| <I/σ(I)> | 9.21 | 1.85 |
| Completeness [%] | 98.5 | 89.1 |
| Redundancy | 4.026 | 3.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 17.5% PEG 3350, 2.5% GLYCEROL, 0.3 M AMMONIUM SULPHATE AND 0.1 M SODIUM ACETATE AT PH 4.6 |
