4UYB
Crystal structure of SEC14-like protein 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Collection date | 2014-01-18 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.243, 83.243, 174.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.120 - 1.500 |
| R-factor | 0.14217 |
| Rwork | 0.140 |
| R-free | 0.17781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1olm |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.571 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.240 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.800 |
| Number of reflections | 98631 | |
| <I/σ(I)> | 20.7 | 2 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 8.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG6000, 0.8M LITHIUM CHLORIDE, 0.1M CITRATE PH 4.2 |
