4UY7
Crystal structure of Histidine bound Histidine-specific methyltransferase EgtD from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2013-08-18 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 |
Unit cell lengths | 74.499, 74.499, 138.954 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.001 - 2.306 |
R-factor | 0.1957 |
Rwork | 0.192 |
R-free | 0.24740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uy5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.153 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.120 | 0.340 |
Number of reflections | 27410 | |
<I/σ(I)> | 39 | 3.7 |
Completeness [%] | 99.4 | 98.4 |
Redundancy | 13.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 0.1 M TRIS PH9.0, 4 M AMMONIUM ACETATE |