4UXE
Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, P21 selenomethionine crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-11 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.840, 76.330, 116.960 |
Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
Resolution | 29.620 - 2.000 |
R-factor | 0.14045 |
Rwork | 0.140 |
R-free | 0.17533 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.343 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.310 |
Number of reflections | 109012 | |
<I/σ(I)> | 12.4 | 5.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 10-16% (W/V) PEG 6000, 12-22% (V/V) GLYCEROL, 0.1 M TRIS-HCL PH 8.5 |